| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress
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| Autores: |
Gonzalez-aravena, Marcelo
Calfio, Camila
Mercado, Luis
Morales-lange, Byron
Bethke, Jorn
De Lorgeril, Julien
Cardenas, Cesar A.
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| Data: |
2018-03
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| Ano: |
2018
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| Palavras-chave: |
Hsp70
Coelomocytes
Antarctica
Echinoderms
Climate change
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| Resumo: |
Background Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequence and the expression of Hsp70 in the Antarctic sea urchin are unknown. Methods We amplified and cloned a transcript sequence of 1991 bp from the Antarctic sea urchin Sterechinus neumayeri, experimentally exposed to heat stress (5 and 10 °C for 1, 24 and 48 h). RACE-PCR and qPCR were employed to determine Hsp70 gene expression, while western blot and ELISA methods were used to determine protein expression. Results The sequence obtained from S. neumayeri showed high identity with Hsp70 members. Several Hsp70 family features were identified in the deduced amino acid sequence and they indicate that the isolated Hsp70 is related to the cognate heat shock protein type. The corresponding 70 kDa protein, called Sn-Hsp70, was immune detected in the coelomocytes and the digestive tract of S. neumayeri using a monospecific polyclonal antibody. We showed that S. neumayeri do not respond to acute heat stress by up-regulation of Sn-Hsp70 at transcript and protein level. Furthermore, the Sn-Hsp70 protein expression was not induced in the digestive tract. Conclusions Our results provide the first molecular evidence that Sn-Hsp70 is expressed constitutively and is non-induced by heat stress in S. neumayeri.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00435/54619/56019.pdf
DOI:10.1186/s40659-018-0156-9
https://archimer.ifremer.fr/doc/00435/54619/
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| Editor: |
Soc Biolgia Chile
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| Formato: |
application/pdf
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| Fonte: |
Biological Research (0716-9760) (Soc Biolgia Chile), 2018-03 , Vol. 51 , P. 8 (10p.)
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| Direitos: |
The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain De
info:eu-repo/semantics/openAccess
restricted use
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